外源有机物添加种类对农田黑土有机碳激发效应的影响及作用机理

李娜, 滕培基, 雷琬莹, 龙静泓, 李禄军

李娜, 滕培基, 雷琬莹, 龙静泓, 李禄军. 外源有机物添加种类对农田黑土有机碳激发效应的影响及作用机理[J]. 中国生态农业学报 (中英文), 2023, 31(10): 1588−1601. DOI: 10.12357/cjea.20230234
引用本文: 李娜, 滕培基, 雷琬莹, 龙静泓, 李禄军. 外源有机物添加种类对农田黑土有机碳激发效应的影响及作用机理[J]. 中国生态农业学报 (中英文), 2023, 31(10): 1588−1601. DOI: 10.12357/cjea.20230234
LI N, TENG P J, LEI W Y, LONG J H, LI L J. Effects and mechanisms of addition of different types of exogenous organic materials on priming effect of organic carbon in arable black soils[J]. Chinese Journal of Eco-Agriculture, 2023, 31(10): 1588−1601. DOI: 10.12357/cjea.20230234
Citation: LI N, TENG P J, LEI W Y, LONG J H, LI L J. Effects and mechanisms of addition of different types of exogenous organic materials on priming effect of organic carbon in arable black soils[J]. Chinese Journal of Eco-Agriculture, 2023, 31(10): 1588−1601. DOI: 10.12357/cjea.20230234

外源有机物添加种类对农田黑土有机碳激发效应的影响及作用机理

基金项目: 中国科学院前沿科学研究重点计划项目(ZDBS-LY-DQC017)、中国科学院战略性先导科技专项子课题(XDA28010301)、国家自然科学基金项目(42277355)、国家重点研发项目(2021YFD1500105-1)和吉林省自然科学基金项目(YDZJ202201ZYTS517)资助
详细信息
    通讯作者:

    李娜, 主要研究方向为土壤肥力调控和有机质生物化学循环。E-mail: nal@iga.ac.cn

  • 中图分类号: S153.6; S154.1

Effects and mechanisms of addition of different types of exogenous organic materials on priming effect of organic carbon in arable black soils

Funds: This study was supported by the Research Program of Frontier Sciences of Chinese Academy of Sciences (ZDBS-LY-DQC017), the Strategic Priority Research Program of Chinese Academy of Sciences (XDA28010301), the National Natural Science Foundation of China (42277355), the National Key Research and Development Program of China (2021YFD1500105-1) and the Natural Science Foundation of Jilin Province (YDZJ202201ZYTS517)
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  • 摘要: 外源有机物添加通过激发效应影响土壤有机碳(SOC)的矿化和稳定。目前研究较少考虑不同种类外源有机物添加对同一土壤类型不同SOC含量的土壤激发效应及作用机理。本研究以中国东北典型黑土带上4个不同SOC含量的表层农田黑土为研究对象, 向土壤中添加13C标记的玉米秸秆、葡萄糖和丙氨酸, 研究不同种类外源有机物添加对黑土SOC激发效应的影响及作用机理。研究发现, 与不添加外源有机物处理相比, 外源有机物添加均促进了4个不同SOC含量的农田土壤中SOC的矿化, 并引起土壤正激发效应。添加葡萄糖和丙氨酸处理的4个土壤在培养前30 d累积激发量均显著大于添加秸秆处理; 土壤本底SOC含量越高的土壤, 添加丙氨酸和葡萄糖产生的累积激发量越大, 但相对累积激发量越小。随着培养时间增加, 4个土壤的SOC矿化和激发效应降低并趋于稳定且处理间无显著差异。傅里叶红外光谱发现, 外源有机物添加后4个土壤脂肪族碳官能团的相对峰面积略有增加, 芳香族碳官能团峰面积略有下降, 土壤和外源有机物添加种类对脂肪族和芳香族碳官能团的影响均表现为土样>添加物种类。结构方程模型表明, 外源有机物添加产生的正激发效应主要受土壤细菌和放线菌磷脂脂肪酸的影响, 受土壤SOC含量影响较小。该研究结果表明, 外源有机物添加显著增加了农田黑土中k型微生物生长代谢, 促进了微生物对SOC中难分解组分的降解, 外源有机物与土壤自身有机碳分解的“共代谢”理论是本研究黑土产生正激发效应的主要作用机理。

     

    Abstract: The addition of exogenous organic matter (EOM) affects the mineralization and stabilization of soil organic carbon (SOC) via priming effects (PE). However, few studies have considered the effects of different EOM additions on PE in the same soil type with a gradient in SOC content. The underlying mechanisms have rarely been revealed, and related studies can provide in-depth insights into the microbial mechanisms that regulate carbon accumulation and stability in agricultural soils. It is crucial to predict dynamic changes in SOC and carbon pool stability in response to EOM inputs from different sources. Therefore, this study focused on topsoil with four SOC contents, which ranged from 10 g·kg1 to nearly 70 g·kg1, in the typical black-soil region of Northeast China, and aimed to investigate the effects and microbial mechanisms involving different types of EOM addition on PEs by adding 13C-labeled maize straw, glucose, and alanine to the soil. Compared to a control treatment without EOM, the addition of EOM promoted the mineralization of SOC in the four soils with different SOC contents. Specifically, glucose, alanine, and straw addition increased the cumulative mineralization of SOC by 50.88%–419.65%, 69.54%–409.48%, and 13.14%–321.43%, respectively. The addition of the three types of EOM also induced a positive PE in soils with different SOC contents. During the initial 30 days of incubation, the cumulative PEs in soils with different SOC contents under glucose and alanine addition treatments were considerably higher than those under straw addition treatment. Soils with higher SOC content exhibited greater cumulative mineralization and PEs with the addition of glucose and alanine, whereas their relative cumulative PEs were lower. SOC mineralization and PEs decreased and reached a stable state with incubation time in soils with different SOC contents. Fourier-Transform Infrared spectroscopy revealed a slight increase in the relative peak area of aliphatic carbon functional group and a slight decrease in the peak area of aromatic carbon group in soils with different contents of SOC after addition of EOM. The effects of SOC content on aliphatic and aromatic carbon functional groups were greater than those of the EOM type. Correlation analysis revealed that cumulative SOC mineralization and PEs were significantly positively correlated with total phospholipid fatty acids, biomass of total bacteria, gram-positive bacteria, gram-negative bacteria, and actinomycetes, with a peak area of aliphatic carbon at 1420 cm1 (P<0.05). In addition, cumulative SOC mineralization and cumulative PEs were significantly negatively correlated with the biomass of fungi and anaerobic bacteria, with a peak area of aromatic carbon at 1630 cm1 (P<0.05). Structural equation modeling indicated that the positive PE resulting from EOM addition was primarily influenced by bacterial and actinomycete phospholipid fatty acids in the soil, regardless of the SOC content of the four soil samples. These results demonstrated that EOM addition significantly increased the growth and metabolism of k-type microorganisms, such as gram-negative bacteria and actinomycetes, in arable black soil and promoted the decomposition of recalcitrant components in SOC. The “co-metabolism” theory, namely the co-decomposition of EOM and SOC, is considered as the primary mechanism behind the positive PE in black soil.

     

  • 图  3   不同种类外源有机物添加对农田黑土有机碳的激发效应(A-D)、培养30 d的累积激发效应(E-H)和相对累积激发效应(I-L)的影响

    Figure  3.   Effects of addition of different types of exogenous organic materials on priming effect (A-D), cumulative priming effect (E-H) and relative cumulative priming effect (I-L) during the first 30 days of incubation of arable black soils with different soil organic carbon contents

    图  1   4个供试土壤主要有机碳官能团的相对峰面积

    Figure  1.   Relative peak areas of main organic carbon functional groups of four bulk soils determined by Fourier Transform Infrared Spectroscopy

    图  2   不同种类外源有机物添加对不同土壤有机碳含量的农田黑土有机碳矿化速率(A-D)和累积矿化量(E-H)的影响

    Figure  2.   Effects of addition of different types of exogenous organic materials on mineralization rate (A-D) and cumulative mineralization (E-H) of soil organic carbon of arable black soils with different soil organic carbon contents

    图  4   培养结束(135 d)后不同外源有机物添加对农田黑土有机碳官能团相对吸收峰面积的影响

    Figure  4.   Effects of addition of different types of exogenous organic materials on relative peak areas of organic carbon functional groups in arable black soils after 135 d incubation

    图  5   培养30 d不同外源有机物添加对不同有机碳含量农田黑土微生物群落组成的影响

    Figure  5.   Impacts of addition of different types of exogenous organic materials on composition of microbial community in arable black soils with different exogenous organic carbon contents after 30 d incubation

    图  6   土壤有机碳矿化激发效应与土壤碳官能团和微生物群落组成相关性分析

    Figure  6.   Correlation analysis of soil organic carbon mineralization and priming effect, soil carbon functional groups, and microbial community composition

    图  7   不同外源有机物添加对不同有机碳(SOC)含量农田黑土激发效应影响的结构方程模型(SEM)

    Figure  7.   Structural equation model (SEM) for the effect of different exogenous organic materials addition on soil priming effect in arable black soils with different soil organic carbon (SOC) contents

    表  1   试验前供试土壤基本理化性质和微生物生物量碳含量

    Table  1   Physical-chemical properties and microbial biomass carbon content before incubation of the tested soil

    土样
    Soil sample
    经纬度
    Longitude,
    latitude
    总有机碳
    Soil
    organic carbon
    (g·kg−1)
    全氮
    Total
    nitrogen (g·kg−1)
    pH全磷
    Total phosphorus (g·kg−1)
    全钾
    Total
    potassium (g·kg−1)
    有机磷
    Olsen phosphorus
    (mg·kg−1)
    速效钾
    Available potassium (mg·kg−1)
    微生物生物
    量碳
    Microbial biomass carbon
    (mg·kg−1)
    SOC6848°15′N, 126°21′E 68.16±0.33a 5.65±0.02a 6.89±0.01a 1.73±0.00a 16.2±0.9b 104.4±2.32a 628.8±15.9a 549.72±75.45a
    SOC3247°27′N, 126°55′E31.50±0.22b2.56±0.01b5.63±0.05b1.06±0.02b18.4±0.4a44.5±3.15c138.8±0.8c353.10±4.13b
    SOC2144°59′N, 126°21′E20.59±0.11c2.21±0.12c5.14±0.01c0.97±0.00c18.4±0.3a52.7±2.09b159.0±8.4b117.99±1.13d
    SOC1143°12′N, 124°11′E11.12±0.07d1.07±0.01d5.17±0.01c0.46±0.00d17.5±2.8ab15.6±0.99d106.2±19.3d182.99±23.58c
      SOC68、SOC32、SOC21和SOC11分别表示北安、海伦、榆树和四平采集的土样。同列不同小写字母表示4个土样在P<0.05水平差异显著。SOC68, SOC32, SOC21 and SOC11 represent soils sampled from Bei’an, Hailun, Yushu and Siping, respectively. Different lowercase letters in the same column indicate significant differences among different soil samples (P<0.05).
    下载: 导出CSV

    表  2   土壤有机碳含量和外源有机物种类对土壤有机碳累积矿化量、累积激发量和有机碳官能团相对峰面积影响的双因素方差分析

    Table  2   Two-way ANOVA for the influences of soil organic carbon (SOC) content, addition of different types of exogenous organic materials and their interactions on cumulative SOC mineralization, cumulative priming effect and infrared relative peak area of SOC functional groups

    要素
    Elements
    SMS×M
    PEtaPEtaPEta
    培养135 d累积矿化量
    Cumulative SOC mineralization after 135 d incubation
    <0.001 0.474 <0.001 0.819 <0.001 0.679
    培养30 d累积激发量
    Cumulative priming effect after 30 d incubation
    <0.0010.568<0.0010.2150.0210.147
    培养30 d相对累积激发量
    Relative cumulative priming effect after incubation for 30 d
    <0.0010.624<0.0010.3470.0280.165
    F2920<0.0010.8010.0330.090.0170.189
    F2850<0.0010.7770.0670.0720.0010.255
    F1630<0.0010.9240.8830.0030.2330.103
    F1420<0.0010.8310.0390.0860.0010.264
      S和M分别表示土壤有机碳含量和添加外源有机物种类。P和Eta分别表示P值和偏Eta方。F2920、F2850、F1630和F1420分别表示有机碳官能团在2920 cm−1、2850 cm−1、1630 cm−1和1420 cm−1处的相对吸收峰面积。S and M represent SOC content and type of exogenous organic material addition, respectively. P and Eta represent P value and partial Eta squared, respectively. F2920, F2850, F1630 and F1420 indicate the relative peak absorption areas of organic carbon functional groups at 2920, 2850, 1630 and 1420 cm−1, respectively.
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-04-29
  • 修回日期:  2023-07-04
  • 录用日期:  2023-07-04
  • 网络出版日期:  2023-07-20
  • 刊出日期:  2023-10-15

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