Abstract: Culturing the stems of Malus zumi in vitro,the key techniques including medium,auxin,age of sprouts and agar are studied.The results show that if there is the advanced medium MS+6-BA 0.5mg/L+IAA0.2mg/L for multiplicating ，1/2MS+IAA0.5mg/L or 1/2MS+IBA0.5mg/L for rooting,sprout's age being 30～40 days,agar corresponding to the international standard and the light intensity sustaining at 1500～2000lx,the coefficient of multiplication,the quantity of effictive plantlets and the rate of rootage and growth are best,and the surviving ratio of transplantation of plantlets and nursery seedling is above 90% and 85% respectively,and 1-yr-old tree height is above 1.5m.Based on the study,the industrializing technology of tube-plantlets of Malus zumi and the culture standard which improves a theoretical basis for vegetive propagation of others plant are summarized.