Abstract: Progress on Taxus chinensis var.mairei tissue culture is reviewed in this paper. The paper discusses the effects of explants,culture medium,growth regulator, carbon resources, light condition and small molecular additive on the induction and multiplication of Taxus chinensis var.mairei callus in tissue culture and suggests optimal alternatives. Paclitaxe production is discussed from the view of cell secondary metabolism and application of bio-reactor to Taxus chinensis var.mairei cell suspension culture. Finally, the problems and development direction of Taxus chinensis var.mairei tissue culture research are advanced.