Identification of two cross combinations of F₁ Solanum tuberosum by SSR molecular marker

Cross breeding has been a major breeding method of potato and F₁ hybrid generation authenticity identification critical for objectively obtaining individual plant characteristics. To raise new potato (Solanum tuberosum) varieties with good quality, high yield, strong disease resistance and drought resistance, "J07-6" and "Longshu No.3" were respectively cross bred with "Favorita" and F1 hybrids derived. A total of 86 individual F1 hybrid authenticity of two hybrid combinations of "Favorita" × "J07-6" and "Favorita" × "Longshu No.3" were identified using the SSR molecular markers. Out of 43 pairs, 2 pairs of SSR primers (STM1049 and S7) were selected and used in marker analysis of the hybrid and parent crops. The banding patterns of "Favorita" × "J07-6" and "Favorita" × "Longshu No.3" hybrids were divided into four groups - complementary pattern of parents, deletion pattern, male specific pattern and female specific pattern. A total of 34 individual F₁ hybrids of "Favorita" × "J07-6"and 27 individual F₁ hybrids of "Favorita" × "Longshu No.3" were authentically identified. This showed that SSR molecular marker technology was feasible for potato hybrid authenticity identification. The results of this study laid the needed reference for hybrid breeding of excellent potato strains.