Research of the hairy-root of Rhodiola sachalinensis A．Bor．

The hairy-root culture system of Rhodiola sachalinensis A ．Bor was established in this paper．The results show that the hairy-root can be arisen at cotyledon and cotyledonary node infected by A4，R1601，ATCC15834 agrobacterium Ri-plasmid．There is about 500bp-rolC gene detected from the hairy-root culture by PCR amplifying． The hairy-root grows well at 1／2MS_o liquid medium，scatter light，20℃ and pH 4．5～4．8．In the condition of the lower nitrogen substance．such as NH₄⁺：N0₃^- equal to 1：1，the hairy-root grows well and the content of salidrls-ide has been increased．