Effect of medium composition on the growth and secretion of cultured root of Astragalus adsurgens Pall
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Abstract
The growth and secretion of cultured root of Astragalus adsurgens Pall.are affected by the medium composition.One litre of modified B5medium of stimulating the growth of the cultured root of A.adsurgens contains KNO32660.5mg,(NH4)2SO4 84.1mg,CaCl2·2H2O 75.0mg,MgSO4·7H2O 125.0mg,FeSO4·7H2O 13.9mg,MnSO4·4H2O 5.0mg,α-Naphthylacetic acid(NAA)0.6mg.One litre of modified B5 medium of high-stayer-producing A.adsurgens root culture consists of KNO34565.9mg,(NH4)2SO448.1mg,MnSO4·4H2O5.0mg,inositol 150.0mg,nicotinic acid 1.5mg,vitamin B6 hydrochloride 1.5mg,vitamin B1 15.0mg,NAA0.8mg,and sucrose 50.0g.
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